青稞响应燕麦镰孢菌侵染的转录组分析

Transcriptome analysis of naked barley in responseto Fusarium avenaceum infection

摘要:

为了明确青稞与燕麦镰孢菌在转录水平上的互作,以抗病青稞NQK-01-03和感病青稞甘青2号为材料,以Illumina HiSeq Xten为平台,对抗感病青稞在接菌前、接菌20 d 和40 d后茎基部的转录组进行测序。结果表明:经过测序质量控制,抗感病青稞茎基部共得到112.97Gb Clean Data。DEGs基因表达注释共获得差异表达基因4 280个,其中,上调基因2 037个,下调基因2 243个。通过BLAST软件与COG、GO、KEGG、KOG、NCBI-NR、Pfam、Swiss-Prot 和eggNOG 数据库进行比对,28 869条Unigene 获得注释信息,NCBI-NR数据库注释到的Unigene数量最多达到3 981条,占全部注释基因的13.79%。差异表达基因的KEGG通路富集Q-value≤0.05主要有5条,分别为苯丙氨酸代谢、异喹啉类生物碱的生物合成、酪氨酸代谢、苯丙基类生物合成和光合作用天线蛋白。抗病青稞NQK-01-03和感病青稞甘青2号之间GO 注释系统包含生物学过程、细胞组分和分子功能3个主要分支。eggNOG和NCBI-NR注释的新基因数目最多分别达到1 833和2 396个,所占比例分别为59.22%和77.42%。

Abstract:

In order to clarify interaction mechanism between naked barley and Fusarium avenaceum ,two varieties,NQK-01-03 and Ganqing Ⅱ,were used as materials and Illumina HiSeq Xten was used as the platform tosequence the transcriptome of stem base part before inoculation,20 days and 40 days after inoculation.After thequality control of sequencing,112.97 Gb Clean data were obtained from the stem base part of naked barley.Through the annotation of gene expression,a total of 4280 DEGs were obtained,of which 2 037 were up-regulatedand 2 243 were down-regulated.The annotated information was obtained by comparing the BLAST softwarewith the database COG,GO,KEGG,KOG,NCBI-NR,Pfam,Swiss-Prot and eggNOG database.The most annotatedUnigene in the NCBI-NR database was 3981,accounting for 13.79% of the total annotated genes.Therewere 5 enrichment metabolic pathways with Q-value ≤ 0.05 threshold.The KEGG pathway of differentially expressedgenes mainly concentrated on phenylalanine metabolism,isoquinoline alkaloids biosynthesis,tyrosinemetabolism,phenylpropyl biosynthesis and photosynthesis antenna proteins.GO annotation system includedthree main branches,biological process,cellular component and molecular function.The number of new genes annotated by eggNOG and NCBI-NR were 1833 and 2396 respectively,accounting for 59.22% and 77.42%.Theinteraction mechanism between naked barley and F.avenaceum was preliminarily revealed at the transcriptionallevel.