Abstract:Five rhizobium strains (code: 9,8-3,5-1,18-1 and Z12.3) and 5 Medicago cultivars (M .sativacv.Zhaodong,M .sativa cv.Dongmu No.1,M .sativa cv.Gongnong No.1,M .sativa cv.Nongjing No.8,M .varia cv.Caoyuan No.2 and M .varia cv.Caoyuan No.3) were used to study the best matching combination ofrhizobium-Medicago for sandy land in Horqin.The results showed that plant heights of 5 combinations,includingZhaodong -(9),Nongjing No.8 -(8-3),Dongmu No.1 -(5-1),Caoyuan No.3-(5-1) and Gongnong No.1 -(18-1),were significantly higher than control;nodule weights of 2 combinations,including Nongjing No.8 -(8-3) and Caoyuan No.3 -(5-1),were significantly higher than control;nodules of 3 combinations,including Zhaodong-(9),Zhaodong -(18-1) and Caoyuan No.3 -(5-1) increased significantly compared with control;the numberof branches per unit area in combinations of Dongmu No.1 -(5-1),Gongnong No.1 -(18-1) and CaoyuanNo.3 -(5-1) increased significantly compared with control;the hay yield in combinations of Caoyuan No.2 -(18-1) and Gongnong No.1 -(18-1) increased significantly (19.1% and 13.5%) compared with control.But after inoculation,the height,number of nodules and yield of some varieties were lower than control.And this might becaused by the unmatched symbiosis.The combinations of Caoyuan No.2-(18-1) and Gongnong No.1-(18-1)were the best.