温度及培养基对黄花棘豆种子萌发及幼苗生长的影响

Effectsoftemperatureandmediumonseed germinationandseedlinggrowthof Oxytropisochrocephala

摘要:

采用物理方法剥除黄花棘豆(Oxytropisochrocephala)种皮,设置7个温度梯度以及选用7 种常用植物组织培养基进行黄花棘豆种子萌发及幼苗生长的最佳培养温度和培养基的筛选。结果表明:温度对黄花棘豆种子发芽的影响较大,在10~40℃种子发芽率呈先上升后下降趋势,当温度为25℃ 时,黄花棘豆种子发芽率最高,为94.72%,当培养温度为30℃时,幼苗茎叶长、根长以及两子叶间宽度均达到最大值,其次以25℃培养各指标较高;黄花棘豆种子发芽率最高的是 N6培养基,其次为 MS、 MT;幼苗的两子叶宽度最大的是 LS培养基,幼苗长度最长的是 White培养基,其次为SH 和 N6,幼苗根长最佳培养基为 MT,其次为 White、LS、SH 培养基。结合主成分分析,幼苗生长情况以及培养基配方用来最终确定黄花棘豆种子萌发及幼苗生长最佳培养基为 MS培养基。

Abstract:

InordertoprovideseedlingsfortheregenerationsystemofOxytropisochrocephala,thephysical methodswereusedtoremovetheseedcoatsofO.ochrocephala,andthedifferenttemperaturesandculturemedium wereusedtoidentifythefactorsthataffectseedgermination.Theresultsshowedthatthetemperature wasthesignificanteffectonseedgerminationrate.From10℃ to40℃,theseedgerminationrateincreasedat firstandthendecreasedwithtemperature,thehighestgerminationrate(94.72%)occurredat25℃.Theseedlinglength,rootlengthandwidthbetweenthetwocotyledonsreachedthemaximumat30℃,followedby25℃. Asforthemedium,thehighestseedgerminationratewasobtainedinN6,followedbyMSandMT.Thelargest cotyledonwidthofseedlingwasfoundinLS.Thelongestseedlinglengthwasobservedin White,followedby SHandN6.ThebestmediumforseedlingrootlengthwasMT,followedbyWhite,LSandSH.Combinedwith principalcomponentanalysis,seedlinggrowthandmediumformula,theoptimum mediumfortheseedgerminationandseedlinggrowthwasMSmedium.