青海省高寒草地青藏苔草根围土壤细菌拮抗功能评价及鉴定

Evaluation antagonistic function and identificationof bacteria isolated from rhizospheric soilof Carex alnotificifolia in alpinegrassland in Qinghai Province

摘要:

从青海高寒草地青藏苔草根围土壤样品中分离到8株菌株,其中1T5在28℃下经几次转接后不能生长,将其他7株细菌进行了拮抗功能评价及鉴定。结果表明:7株菌株中,只有1T4和1T8对指示菌有良好的抑菌作用,占分离总株数的25%,抑菌率在54.3%~75.7%,其中1T4对番茄早疫病菌(Alternaria solani)、马铃薯坏疽病菌(Phoma foveata)、马铃薯炭疽病菌(Colletotrichum coccodes)和马铃薯枯萎病菌(Fusarium avenaceum )的抑菌率分别为67.1%、61.4%、75.7%和69.2%;1T8对番茄早疫病菌及马铃薯坏疽病菌的抑菌率分别为65.7%和54.3%,对马铃薯炭疽病菌和马铃薯枯萎病菌没有抑菌作用;1T1、1T2、1T3、1T6和1T7对4种指示菌均没有拮抗作用;根据培养性状和形态特征,结合16S rDNA 基因序列分析,将1T4鉴定为解淀粉芽孢杆菌(Bacillus amyloliquefaciens),1T8鉴定为腐生葡萄球菌(Staphylococcus saprophyticus)。

Abstract:

Eight bacteria strains were isolated from the rhizospheric soil of Carex alnotificifolia in alpinegrassland in Qinghai Province.Among them,1T5 could not grow after several transfers at 28℃.The antagonisticfunction of the other 7 strains of bacteria was evaluated and identified.The results showed that only 1T4 and1T8 performed good inhibitory effect on the indicator fungi,accounting for 25% of the total isolates,and the inhibitoryrate ranged from 54.3% to 75.7%.In which,the inhibition ratios of 1T4 on Alternaria solani,Phomafoveata,Colletotrichum coccodes and Fusarium avenaceum were 67.1 %,61.4%,75.7% and 69.2%,respectively.The inhibition ratios of 1T8 against A .solani and P .foveata were 65.7% and 54.3%,1T8 had no inhibitoryeffect on C.coccodes and F.avenaceum ,respectively;Other bacteria,like 1T1,1T2,1T3,1T6 and 1T7 hadno antagonistic effect on 4 indicator fungi;According to the culture characteristics and morphological characteristics,combining with 16S rDNA gene sequence analysis,the soil bacteria 1T4 was identified as Bacillusamyloliquefaciens,1T8 as Staphylococcus saprophyticus.