Abstract:Different levels of N fertilizer were applied to the sown Leymus chinensis pasture (0,N0;100,N1;200,N2;300,N3 and 400 kg/ha,N4) to study the effect of nitrogen application on unstructured carbon and nitrogenin leaf of Leymus chinensis in sandy land.The result showed that the nitrogen application increased thesoluble sugar content.With the increase of nitrogen application level,the starch content in the upper leaf decreased,and it increased first and then decreased in the lower leaf in the second cut.Nitrogen application significantlyincreased the content of soluble protein (P <0.05),and the content of free amino acids in the first cut.The leaf C/N showed a trend of decreasing first and then increasing with the increase of nitrogen applicationlevel.The leaf C/N was the lowest under N1 treatment in upper leaf.It could be concluded that N1 treatmentwas the best.