紫花苜蓿‖玉米间作对玉米氮代谢相关酶活性的影响

Effect of alfalfa‖maize intercropping on nitrogenmetabolism related enzyme activity of maize

摘要:

通过营养液砂培模拟试验,对紫花苜蓿‖玉米间作在不同氮素水平、不同种植方式下玉米氮代谢相关酶活性和氮代谢产物进行研究。结果表明:玉米在不同氮素水平下地上部和地下部的NR、NiR、GS和GOGAT活性、干物质重和氮积累量均随生育时期的推进而不断增加。玉米氮代谢酶活性和氮代谢产物的各指标在N210下均高于N21,并且不同种植方式下其均表现为不分隔>尼龙网分隔>塑料分隔>玉米单作。NR、NiR、GS和GOGAT活性以及氮含量在各生育期均表现为不分隔时显著大于塑料分隔与玉米单作(P <0.05)。干物质重及氮积累量在拔节期和孕穗期表现为不分隔显著大于塑料分隔和玉米单作(P <0.05)。紫花苜蓿‖玉米间作可以提高玉米氮代谢相关酶活性以及氮代谢产物的积累。紫花苜蓿‖玉米中根系互作越紧密、氮素浓度越高,越有利于玉米的氮代谢相关酶活性的提高以及氮代谢产物的积累;氮代谢相关酶活性可以直接反应其氮代谢的能力。

Abstract:

In order to explore the effects of different intercropping systems on nitrogen metabolism enzymesactivities and nitrogen metabolites of maize,the changes of nitrogen metabolism enzymes activities and nitrogenmetabolites at different nitrogen levels were studied.The results showed that NR,NiR,GS,GOGAT,dry matterand nitrogen accumulation of the shoot and root of maize were increased along with the growth stage.Under dif-ferent nitrogen levels,NR,NiR,GS,GOGAT,dry matter,nitrogen content and nitrogen accumulation of theshoot and root of maize with no-barrier were significantly larger than those of plastic barrier and sole maize (P<0.05).NR,NiR,GS and GOGAT under normal nitrogen level were larger than those under low nitrogen level,and those with under-ground interaction were larger than those with above-ground interaction.Dry materweight of maize at seeding stage had no significant difference between no barrier,nylon barrier and plastic barrier.Dry weight at jointing stage and booting stage,nitrogen content and nitrogen accumulation at 3 growth stageswith no barrier were higher than those with plastic barrier and sole maize.It could be concluded that alfalfa‖maize intercropping system could increase the nitrogen metabolism enzymes activities and nitrogen metabolites.And higher nitrogen level promoted the nitrogen metabolism enzymes activities and nitrogen metabolitesaccumulation of maize in alfalfa‖maize intercropping.The activity of nitrogen metabolism enzymes directly reflectedthe nitrogen metabolic capability.