接种红色荧光蛋白标记根瘤菌对苜蓿幼苗生长及结瘤能力的影响

Effectof the red fluorescent marked rhizobiasanfalfaseeding growth and nodulation ability

摘要:

将含有红色荧光蛋白(RFP)基因质粒的荧光标记根瘤菌S.LZgn5-rfp2,S.12531-rfp4、S.LH343-rfp3接种于甘农5号和WL343HQ紫花苜蓿,分别测定2个苜蓿品种幼苗的生物量、生长指标、固氮酶活性等指标。结果表明:RFP标记根瘤菌与出发菌相比对苜蓿幼苗生长无显著影响,与不接菌的苜蓿幼苗相比有明显的促生效果,能稳定的在苜蓿幼苗体内表达,并具有与出发菌无差异的促生能力,2个苜蓿品种间也无特异性。

Abstract:

Red fluorescent protein marked rhizobias (S.LZgn5-rfp3、S.LZgn5-rfp4、S.LZgn5-rfp2,S.12531-rfp3、S.12531-rfp4、S.12531-rfp2、S.LH343-rfp3、S.LH343-rfp4、S.LH343-rfp2) that contained the plasmid with RFP (red fluorescent protein) gene were inoculated to Gannong No.5 alfalfa and alfalfa WL343HQ.Biomass,nodule number,nitrogenase activity and the rate of effective nodules were measured.The results showed that the RFP fluorescence-marked rhizobias could promote plant growth and biomass accumulation of inoculated alfalfa.Compared with their corresponding original rhizobias,the RFP fluorescence-marked rhizobias did no injury the alfalfa seedling,could be expressed consistently in alfalfa plant,had no difference from the starting bacteria,had the similar growth promotion ability with original rhizobias.It could be stably expressed in alfalfa seedlings and had no difference from the starting bacteria.Considering ability,there is no specificity between the two alfalfa varieties.