高寒阴湿区6个抗寒紫花苜蓿种质氨基酸积累量分析

Analysis of amino acid accumulation of six coldresistant alfalfa strains in cold and humid area

摘要:

为了评价6个抗寒紫花苜蓿种质(GNKH-1、2和3、俄罗斯西伯利亚杂花苜蓿、金皇后紫花苜蓿和阿尔冈金紫花苜蓿)的种质,在青藏高原高寒阴湿区进行了播种当年的评比试验,并对地上部分叶和茎中的氨基酸含量进行分析。结果表明:氨基酸总量(T)在GNKH-2叶最高(23.86%),在俄罗斯西伯利亚杂花茎中最高(12.13%);必需氨基酸(E)在金皇后叶(8.55%)和茎(4.27%)中最高。供试苜蓿叶蛋白种质从高到低排序为:金皇后＞GNKH-1＞GNKH-3＞GNKH-2＞阿尔冈金＞俄罗斯西伯利亚杂花;苜蓿茎蛋白品质从高到低排序为:金皇后＞GNKH-1＞GNKH-2＞GNKH-3＞俄罗斯西伯利亚杂花＞阿尔冈金。综合分析表明,抗寒苜蓿材料中GNKH-1叶和茎蛋白质价值均最高;GNKH-3是抗寒苜蓿材料中氨基酸评分相对最高的,叶部氨基酸评分(AAS)为32.74,茎部为15.94,均高于其他供试苜蓿品系;阿尔冈金和GNKH-1叶部EAAI值最高,分别为1.2860和1.2847;GNKH-2(1.2872)仅次于俄罗斯西伯利亚杂花和阿尔冈金茎部的EAAI值(1.2881,1.2876)。

Abstract:

order to evaluate the quality of six cold-resistant alfalfa strains (GNKH-1, GNKH-2, GNKH-3,Medicago sativa subsp varia,M. sativa cv. Golden Empress and M. sativa.cv.Algonguin), a comparison experiment was conducted in the year of sowing in the cold and humid area of Qinghai-Tibet plateau,and the contents of amino acids in the above-ground parts(leaves and stems)were analyzed. Result showed that the highest contents of total amino acids were observed in leaf of GNKH-2 (23.86%) as well as in stem of M. sativa subsp. varia (12.11%). Both the leaf and stem of M. sativa cv. Golden Empress contained the most essential amino acids, with each reaching 8.55% and 4.30%, respectively. The protein quality priority in leaf tended to decrease in the ordor M. sativa cv. Golden Empress＞GNKH-1＞GNKH-3＞GNKH-2＞M. sativa. cv. Algonguin＞M. sativa subsp.varia and that in stem was M. sativa cv. Golden Empress＞GNKH-1＞GNKH-3＞GNKH-2＞M. sativa subsp. varia＞M. sativa cv. Algonguin. Comprehensive analysis showed that the leaf and stem of GNKH-1 gave the highest protein values;GNKH-3 ranked number 1 in terms of amino acid score (AAS), reaching 32.74 in leaf and 16.24 in stem; the essential amino acids index (EAAI) in leaf of GNKH-1 (1.279 9) and M. sativa cv. Algonguin (1.280 9) exceeded that of all other tested materials;EAAI in stem of GNKH-2 (1.281 1) was only 0.52% lower than that of Algonquin (1.287 8), which presented the highest EAAI in all tested materials.