Abstract:This study was conducted to examine the effects of physiological and biochemical characteristics of five forages under high temperature stress (38℃/30℃).The relative enzyme activity,malondialdehyde content,chlorophyll content and other physiological indexes were determined under high temperature stress.The results showed that under high temperature stress,the activities of superoxide dismutase (SOD) and peroxidase (POD),the content of soluble protein (SP) and glutathione (GSH) of five forage species showed a trend of first rising and then declining,while the content of malondialdehyde (MDA) continued to rise,the content of chlorophyll (SPAD) showed a significant decline.The adaptability of Paspalum notatum Flugge and Paspalum wetsfeteini to high temperature stress was significantly better than that of Festuca elata,Lolium multiflorum and Dactylis glomerata.