Abstract:The characteristics of enzyme activity in surface soil of alpine steppe under different altitudes (3094,3227,3470,3901 and 4200 m) in Sanjiangyuan region on Qinghai-Tibetan Plateau were studied in order to explore the variation patterns of activity of soil enzymes (phosphatase,polyphenol oxidase,peroxidase,dehydrogenase).The results showed that the altitude had little effect on the vegetation biomass of alpine steppe,but the increased elevation increased the plant coverage and diversity of steppe,while decreased the grass height.Soil phosphatase activity increased with altitude,while soil polyphenol oxidase,peroxidase and dehydrogenase increased first and then decreased with the increase of altitude gradient.And with the altitude,the soil bulk density,water content and pH decreased,the total nitrogen and organic matter content showed reversed trends,while total phosphorus and potassium showed no significant change.Redundancy analysis showed that pH,organic carbon and moisture contents and the proportion of gramineae in vegetation were the main factors affecting significant effects on soil enzyme activities.