长枝木霉TL16菌株对南方根结线虫的防治效果

Control effect of strain TL16 on Meloidogyneincognita in vitro and by pot experiment

摘要:

为探明TL16菌株对南方根结线虫的防治效果,先利用长枝木霉TL16(Trichoderma longibrachiatum)孢子悬浮液测定对南方根结线虫(Meloidogyne incognita)卵和二龄幼虫的离体作用。进一步采用盆栽试验探讨TL16对黄瓜根结线虫病的防治效果。结果表明:长枝木霉菌株TL16孢子悬浮液抑制线虫卵的孵化(53.58%~69.05%),其中,处理后9 d,7.5×10⁶ cfu/mL的TL16孢子悬浮液对线虫卵孵化的抑制率最高(69.05%)。然而,孢子悬浮液对二龄幼虫无明显的致死作用(0.96%~1.65%)。同时,盆栽试验发现,浓度为7.5×10⁶ cfu/mL的TL16孢子悬浮液对黄瓜根结线虫的防效最佳(55.00%)。

Abstract:

In order to detect the control effect of strain TL16 (Trichoderma longibrachiatum) on Meloidogyne incognita,the spore suspensions of TL16 were used to measure its effects on eggs of M.incognita and 2nd-stage juveniles in vitro.And the pot experiment was conducted to explore the control effect of TL16 on cucumber root-knot nematode disease.The results showed that the spore suspension of TL16 inhibited the hatching of nematode eggs (53.58% to 69.05%),and the inhibition rate of TL16 spore suspension with 7.5×10⁶ cfu/mL on the hatching of nematode eggs was the highest (69.05%) after treated for 9 days.However,the spore suspension had no significant lethal effect on 2nd-stage juveniles (0.96% to 1.65%).At the same time,the pot experiment showed that TL16 spores with 7.5×10⁶ cfu/mL showed the highest control effect on nematodes (55.00%).