中国红豆杉植株再生体系优化

Study on optimization of regeneration systemof Taxus chinensis

摘要:

以中国红豆杉的嫩枝和试管苗为外植体,采用三因子D-饱和最优设计方案,筛选最适合诱导茎段、叶片愈伤组织和分化芽的培养基。结果表明:WPM+6-BA 2.25 mg/L+2-ip 1.5 mg/L+NAA 0.2 mg/L和WPM+6-BA 3.4 mg/L+2-ip 3.0 mg/L+NAA 0.2 mg/L为茎段、叶片最适合诱导愈伤组织的培养基;WPM+6-BA 3.4 mg/L+2-ip 4.5 mg/L为最适合茎段愈伤组织分化芽的培养基;WPM+NAA 0.4 mg/L为适合生根的培养基。

Abstract:

The shoots and test-tube seedlings of Taxus chinensis were used as explants to screen out the medium suitable for inducing callus of stem segments and leaf callus and differentiated shoots through the three-factor saturation-D optimal design. The results showed that WPM + 6-BA 2.25 mg/L+2-ip 1.5 mg/L+NAA 0.2 mg/L was suitable for stem segments callus induction,and WPM+6-BA 3.4 mg/L+2-ip 3.0 mg/L+NAA 0.2 mg/L was suitable for leave callus induction,WPM+6-BA 3.4 mg/L+2-ip 4.5 mg/L was the most suitable medium for stem bud differentiation;WPM+NAA 0.4 mg/L was suitable medium for rooted.