燕麦地上部分提取物对油菜的化感作用研究

Study on allelopathic potential of abovegroundextract from Avena sativa to Brassica juncea

摘要:

以燕麦品种百绿1号地上部分为供体,用80%乙醇、甲醇、水、石油醚和乙腈5种溶剂分别在4℃,15℃和25℃温度下提取燕麦地上部分化感物质,通过生物活性测定法分析不同提取条件下的燕麦地上部分提取物对油菜种子萌发及幼苗生长的化感作用。结果表明:不同溶剂及提取温度下燕麦地上部分提取物的化感潜力差异显著(P＜0.05),各处理均能显著抑制油菜种子萌发和幼苗根的生长,对其芽长的化感效应指数在-0.39 ~ 0.15,既有抑制作用也有促进作用。水提取物、80%乙醇提取物均对油菜种子萌发表现强烈化感作用,但作用强度不及25℃下的甲醇提取物;三者对油菜幼苗生长的影响没有明显规律。

Abstract:

The aboveground part of Avena strigosa cv.Bailv No.1 was used as donor,and 80% ethanol,methanol,water,petroleum ether and acetonitrile were used to extract allelochemicals at 4℃,15℃ and 25℃.The allelopathic effect of the extracts on the seed germination and seedling growth of Brassica juncea under different conditions was analyzed by bioactivity assay.The results showed that the allelopathic potential of the extracts was significantly different under extraction conditions (P＜0.05).All treatments could significantly inhibit the seed germination and seedling root growth of Brassica juncea,and the allelopathic effect index on shoot length ranged from -0.39 to 0.15,which showed both inhibitory and promoting effects.Both water extract and ethanol extract performed strong allelopathic effects on Brassica juncea seed germination,but the effect intensity was less than that of methanol extract at 25℃.