禾谷孢囊线虫生防真菌AT9的固体发酵条件优化

Optimization of solid fermentation conditions of Aspergillus terreus for wheat cyst nematode biocontrol

摘要:

AT9菌是对禾谷孢囊线虫(Heterodera avenae)具有生防作用的真菌。通过固体发酵底物、营养物质以及发酵条件进行优化,以期为该菌生产应用奠定基础。首先,采用正交设计筛选了AT9固体发酵基质。其次,通过单因素及正交设计分析了不同碳、氮源以及无机盐的最优配比。最后,运用单因素方差分析筛选了AT9的最佳发酵条件。结果表明:(1)AT9最佳发酵基质为麦麸、玉米粉、玉米秸秆粉按2∶1∶3质量比混配;(2)最佳碳源、氮源与无机盐比例为4%葡萄糖、6%硝酸钾、2%碳酸钙;(3)固料∶水为 1∶1.2,20%的接种量,32℃黑暗条件培养8 d为最佳发酵条件。

Abstract:

Aspergillus terreus AT9 is a funus with biological control effect against cereal cyst nematode (Heterodera avenae).The solid fermentation substrate,nutrients and fermentation conditions of AT9 was optimized to provide a foundation for its production and application.Orthogonal design was used to screen different substrates for solid fermentation.Single factor and orthogonal design were used to analyze the optimal proportion of different carbon and nitrogen sources.One-way analysis of variance was used to screen the optimal fermentation conditions.The results showed that 1) The optimal fermentation substrate was mixture of wheat bran,corn flour and corn straw powder (2∶1∶3);2) The optimal carbon source,nitrogen source and inorganic salt ratio were 4% glucose,6% potassium nitrate and 2% calcium carbonate;3) The best fermentation conditions were solid material/water ratio 1∶1.2,20% inoculum at 32 ℃ in dark for 8 days.