Abstract:A field experiment was carried out in Agricultural Sciences Base of Qingyang Academy,Gansu Province to evaluate the effects of defoliant (paraquat) application on defoliation rate,stem and leaf rate of water loss,seed yield,seed yield components and seed vigor of alfalfa.The results showed that the different concentrations of defoliant significantly increased the stem and leaf relative water loss rate,and significantly shorten the drying time of alfalfa;0.40% to 0.55% of defoliant concentration were the best,and the defoliation rate reached 34.26% and 34.64% respectively.The defoliant did not affect the alfalfa yield in first year,but the shoot density was decreased in second year.The abnormal seedlings increased with the increasing of defoliant concentration.