Abstract:By using 5 growth-promoting strains previously isolated from plant rhizosphere,a rhizosphere fertilizer formula for alfalfa was developed.And then,the bacterial fertilizer was prepared with 3 forms (solid,GF;liquid,YF;seed coating,BF).A field randomized block experiment was designed with 11 treatments (GF,YF,BF,GF+7/10H,YF+710H,BF+7/10H,GF+1/2H,YF+1/2H,BF+1/2H,CK and H),including 3 forms of bacterial fertilizer and reduced chemical fertilizer (7/10H,1/2H,H),to determine the effects on the growth and quality of alfalfa.Results showed that compared with the control (CK,no fertilizer),GF+7/10H treatment significantly improved the performance of alfalfa production.The plant height was 47.95 cm;The alfalfa hay yield was increased by 47.78%,which was 1.11 times of the full amount of chemical fertilizer treatment;The alfalfa nutritional quality was significantly improved;The relative forage value was 125.87,significantly higher than other treatments.Compared with full amount of chemical fertilizer treatment,the bacterial fertilizer could replace 30% of chemical fertilizer without significant difference in alfalfa production performance and nutritional quality.