The effect of slope gradient on the growth characteristics and slope protection of Medicago sativa roots in the process of vegetation restoration on rocky slopes was studied by investigating the root characteristics of three years old M.sativa growing on 40°,50° and 60° rock slopes using excavating whole root systems method along the Beijing-Chengde Expressway.The pull-out resistance of roots was measured by hydraulic tension meter.The results showed that with the increase of slope gradient,the diameter of taproot tended to increase,the diameter of grade I and grade II lateral roots decreased.With the increase of slope gradient,the length proportion of lateral root tended to increase,taproot decreased,fine root (0 mm<d≤2 mm) increased,medium root (2 mm<d≤5 mm) decreases gradually.The growth direction of taproot did not change with the change of slope.The biomass of lateral root was much less than taproot,and under the condition of 40° slope,the biomass of taproot was significantly less than that of 50 ° and 60 ° slopes (P<0.05).With the increase of slope gradient,the root pull-out resistance increased gradually.