安徽野生黄花苜蓿与紫花苜蓿产量性状、核型及遗传纯合度比较分析

Comparative analysis of yield traits,karyotypic andhereditary homozygosity between wild Medicagofalcata and Medicago sativa in Anhui

摘要:

为探究安徽野生黄花苜蓿的产量和遗传背景,以安徽野生黄花苜蓿和紫花苜蓿巨能601为材料,测定产量性状,分析染色体核型,利用SSR分子标记鉴定基因型。结果表明:黄花苜蓿株高与紫花苜蓿没有显著差异,主茎节数显著多于紫花苜蓿,紫花苜蓿鲜草及干草产量都显著高于安徽野生黄花苜蓿。SSR分子标记检测表明安徽野生黄花苜蓿具有更高的纯合度,其纯合位点占总检测位点的60.20%,巨能601纯合位点仅占总检测位点的41.96%,有2、3和4个等位基因的位点分别占36.61%、13.39%和8.04%,2个品系的遗传相似性为63.39%。

Abstract:

In order to study the yield traits and genetic background of wild Medicago falcata grown in Anhui and Medicago sativa (Magna 601),the comparative analysis was conducted by measuring the yield capability,chromosome karyotype and hereditary homozygosity of two materials.The results showed that there was no significant difference between materials in terms of plant height but the node number of main stem of wild M.falcata was significantly higher than that of M.sativa,and the yield (fresh and hay) of M.sativa was significantly higher than that of wild M.falcata.Wild M.falcata had higher homozygosity than M.sativa (Magna 601).The homozygous loci of wild M.falcata accounted for 60.20% of the total loci and that of M.sativa (Magna 601) accounted for 41.96%,and the loci of 2,3 and 4 alleles accounted for 36.61%,13.39% and 8.04%,respectively.