10个草地早熟禾品种对白粉病的抗性评价及生理特性分析

Resistance evaluation and physiological characteristicanalysis of ten Poa pratensis varietiesto powdery mildew

摘要:

以10个草地早熟禾品种为材料,采用接种法研究了其对白粉病的抗病性,并对相关生理生化指标进行测定。结果表明:不同品种草地早熟禾对白粉病的抗病性存在显著差异,其中黑杰克抗病性较强,其次为午夜2号、Shamrock和公园;而解放者、超级哥来德的抗病性相对较差,超级哥来德最差,达极感程度。聚类分析表明,10个草地早熟禾品种大致分为5类:1)黑杰克为高抗品种;2)午夜2号、Shamrock和公园为中抗品种;3)橄榄球2号、耐力和耐盐月夜为中感品种;4)解放者和抢手股为高感品种;5)超级哥来德为极感品种。生理生化指标测定结果表明,抗病品种接种白粉菌后叶绿素、可溶性糖和可溶性蛋白含量下降幅度较小,感病品种下降幅度较大;抗病品种的丙二醛含量在接菌后上升幅度较小,而感病品种上升幅度较大;抗病品种的游离脯氨酸含量,SOD、POD、CAT、PAL和PPO活性在接菌后显著提高,而在感病品种中均有所下降。

Abstract:

Ten Poa pratensis varieties were used to study the resistance to powdery mildew by resistance inoculation method,and the related physiological and biochemical indexes were determined.The result showed that there were significant differences in powdery mildew resistance among different varieties.Out of 10 tested varieties,Blackjack was the best,which was followed by Midnight 2,Shamrock and Park.The disease resistance of Liberator,Bluechip and EverGlade was relatively poor,in which,EverGlade was the poorest and extremely susceptible to disease.Cluster analysis showed that 10 varieties could be divided into 5 groups: group 1 (Blackjack) was highly resistant,group 2 (Midnight 2,Shamrock and Park) was moderately resistant,group 3 (Rugby 2,Stamina and Salt-tolerant moonlight) was moderately susceptible,group 4 (Liberator and Bluechip) was highly susceptible and group 5 (EverGlade) was extremely susceptible.The results of physiological and biochemical indicators showed that the contents of chlorophyll,soluble sugar and soluble protein in resistant varieties decreased slightly,while those in susceptible varieties decreased significantly after powdery mildew infection;MDA content of resistant varieties increased slightly,while it significantly increased for susceptible varieties;The free proline content,SOD,POD,CAT,PAL and PPO activity of resistant varieties increased significantly,but decreased for susceptible varieties.The research work provides a reference for powdery mildew resistance breeding work of Poa pratensis.