干旱胁迫下丁二醇诱导对匍匐翦股颖幼苗生理生化指标的影响

Effects of butyldiol induction on physiologicaland biochemical characteristics of Agrostisstolonifera under drought stress

摘要:

为了研究丁二醇对匍匐翦股颖(Agrostis stolonifera)苗期抗旱性的调控效应,以匍匐翦股颖(PennA-4)种子为材料,进行沙培种植,待幼苗生长粗壮均匀时进行丁二醇的处理,5天后用15%的PEG进行模拟抗旱处理,分析苗期植株丙二醛(MDA)、可溶性糖、叶绿素、相对含水量、游离脯氨酸、抗坏血酸过氧化物酶(APX)、过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的变化规律及与植物抗旱性的关系。研究结果表明:与干旱胁迫处理相比,诱导处理显著提高了植株叶片相对含水量、脯氨酸以及叶绿素的含量,抑制了匍匐翦股颖中丙二醛(MDA)和可溶性糖的含量,同时提高了超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性,并且使过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)的活性有所降低。综上,在干旱胁迫条件下,外施丁二醇可提高翦股颖的抗旱能力,使其能够更好地适应干旱胁迫环境。

Abstract:

The effect of butanediol on the regulation of drought-resistance at the seedling stage of bentgrass (PennA-4) was studied.The seedlings treated with butanediol for 5 days were used for simulated drought treatment with 15% PEG.The variation of malondialdehyde (MDA),soluble sugar,chlorophyll,relative water content,free proline,ascorbate peroxidase (APX),peroxidase (POD),superoxide dismutase (SOD) and catalase (CAT) was investigated and their relationship with drought resistance was analyzed.The results showed that compared with the drought stress treatment,the relative water content,proline content and chlorophyll content in leaf were significantly increased after induction treatment,but the contents of malondialdehyde (MDA) and soluble sugar were decreased.The activities of superoxide dismutase (SOD) and catalase (CAT) were increased,and the activities of peroxidase (POD) and ascorbate peroxidase (APX) were decreased.Under the drought stress conditions,the butanediol application can improve the drought resistance of creeping bentgrass.