BYDV侵染对燕麦总酚和渗透调节物质含量的影响

Effects of BYDV infection on total phenol andosmotic adjustment substances in oat

摘要:

为探究燕麦在蚜虫和大麦黄矮病毒(BYDV)为害后的生理响应,研究了接种蚜虫和BYDV后不同时间( 0,7,14,21,28,35 d),燕麦叶片的总酚、可溶性糖、可溶性蛋白和脯氨酸含量的变化情况。结果表明:蚜虫为害和BYDV侵染对燕麦总酚和渗透调节物质有显著影响。随着胁迫时间的增加,总酚含量呈明显上升趋势,接毒处理(接种携带BYDV的蚜虫)在接种35 d后较不接毒对照高38.96%(P＜0.05);可溶性糖含量先上升后下降,接种后21 d,接毒处理的可溶性糖含量显著高于接虫处理,且较对照高41.59%。;可溶性蛋白含量显著下降,接种后28 d和35 d,接毒处理的可溶性蛋白含量较接虫处理分别降低了20.40%和15.81%;脯氨酸含量总体逐渐升高,接种后14~21 d,接毒处理脯氨酸含量持续升高并达到最大值,较接虫处理高27.02%(P＜0.05)。

Abstract:

The physiological response of oat to aphid and barley yellow dwarf virus (BYDV) was studied by measuring the contents of total phenol content,soluble sugar,soluble protein and proline in the leaf of infected oat at different dates (0,7,14,21,28 and 35 days).The results showed that aphids and BYDV infection had significant effects on total phenolic and osmotic adjustment substances in oat.With the increase of stress time,the total phenolic content showed a significant increasing trend.And its content in infection treatment (inoculation of aphids carrying BYDV) was significantly higher than that in control (without aphids) by 38.96% after 35 days of inoculation (P＜0.05).The content of soluble sugar first increased and then decreased.After 21 days of inoculation,the difference of the soluble sugar content between the BYDV inoculated treatment and the aphid inoculated treatment was significant,and it was 41.59% higher than the control.The soluble protein content decreased after treatment for 28 and 35 days,and it was 20.40% and 15.81% lower in BYDV treatment than that in aphid treatment.After inoculation for 14 to 21 days,the proline content in BYDV treatment kept increasing to a maximum,which was significantly higher than that in aphid treatment by 27.02% (P＜0.05).