红三叶新品系的抗旱性研究

Study on the drought resistance of new red clover line

摘要:

为评价红三叶新品系(R)的抗旱性,以岷山红三叶(CK1)和甘红1号红三叶(CK2)为对照,采用人工控制干旱胁迫鉴定法,研究在不同程度干旱胁迫下,参试红三叶材料的7项生理生化指标。结果表明:3个红三叶材料中,R的平均丙二醛含量、超氧化物歧化酶和过氧化物酶活性最高,干旱胁迫使红三叶材料的平均叶片含水量和叶绿素含量逐渐降低,可溶性糖和丙二醛含量逐渐升高,叶片超氧化物歧化酶、过氧化物酶及过氧化氢酶活性呈先升高后下降;从红三叶材料与干旱胁迫时间交互作用来看,R的叶片含水量和叶绿素含量变化幅度较小,可溶性糖含量和丙二醛含量变化幅度较大,叶片超氧化物歧化酶和过氧化物酶活性最高。利用隶属函数法对3个红三叶材料的抗旱性综合评价得出,3个红三叶材料的抗旱性顺序依次为:R＞CK2＞CK1。

Abstract:

The drought resistance of new red clover line (R) was studied by using Trifolium pretense cv.Minshan (CK1) and Ganhong No.1 (CK2) as controls,and the leaf water content (LWC),chlorophyll content (CHL),soluble sugar content (SS),malondialdehyde content (MDA),and activities of superoxide dismutase (SOD),peroxidase (POD) and catalase (CAT) in red clover were measured under different degree of drought stress.Results showed that among 3 materials,the MDA content,activities of SOD and POD in R was the highest.The contents of LWC and CHL of 3 materials decreased gradually with the time of drought stress,contents of MDA and SS increased gradually,and activities of POD,SOD and CAT increased first and then decreased.From the interaction of red clover materials and drought stress days,the LWC and CHL of R varied slightly,while the contents of SS and MDA varied greatly,the activity of SOD and POD in leaves was the highest.Comprehensive evaluation based on the membership function method showed that R had the strongest drought resistance,followed by CK2 and CK1.