The lack of high-quality leguminous pasture resources has become a factor that limiting the production and sustainable development of high-quality pastures in the cold regions of northern China.In this study,16 low fall-dormancy alfalfa cultivars were used as materials to study their cold resistance by pot of soil culture.At the same time,principal component analysis and membership function method were used to evaluate the cold resistance,and regression analysis of alfalfa cold resistance and fall dormancy grade was conducted by linear regression method.The purpose of this study was to selected high cold resistant alfalfa cultivars that can be planted in cold areas,and explore the correlation between cold resistance and fall-dormancy grade.The results showed that Longmu 801 and Longmu 806 had the best cold resistance compared to other alfalfa cultivars among the 16 alfalfa cultivars.The cold resistance of Gannong NO.2 was followed Longmu 801 and Longmu 806,and the cold resistance of Juneng 551 was the worst.There was a significant negative correlation between cold resistance and fall dormancy index of alfalfa (R2=0.92),the fall-dormancy was lower,the cold resistance was stronger of alfalfa.Therefore,It has the practical value to predict the cold resistance of alfalfa by using fall-dormancy characteristics in the introduction and production.