苦参碱对二斑叶螨解毒酶活力的影响

The effect of matrine on detoxification enzymes inTetranychus urticae

摘要:

探讨了亚致死剂量苦参碱对二斑叶螨体内解毒酶比活力的影响,以解释二斑叶螨(Tetranychus urticae)对苦参碱的抗性,为苦参碱的科学使用提供理论依据。采用玻片浸渍法,分别用苦参碱的亚致死剂量LC₁₀(0.021 mg/L)和LC30(0.385 mg/L)处理二斑叶螨雌成螨,处理3、6、12、24、48 h后测定分析其体内谷胱甘肽S-转移酶(GSTs)、羧酸酯酶(CarE) 以及多功能氧化酶(MFO)的酶比活力。结果发现:LC₁₀和LC₃₀处理后,与对照相比,随着时间的推移,二斑叶螨雌成螨体内CarE、GSTs和MFO的比活力均有不同程度升高。LC30处理组CarE、MFO (除3 h外) 比活力均高于LC₁₀处理组,在12~24 h比活力最大;GSTs比活力在3、6和12 h的3个时间点LC₁₀处理组高于LC₃₀处理组,且差异显著(P＜0.05),在24 h后达最大。随着苦参碱浓度的增加,CarE、MFO比活力增大,而GSTs比活力无明显变化。

Abstract:

In this study,the effect of sublethal dose of matrine on the activities of detoxification enzymes in Tetranychus urticae was explored to explain the resistance of T.urticae to matrine and provide evidence for scientific using in matrine.The sublethal dose of matrine,LC₁₀ (0.021 mg/L) and LC₃₀ (0.385 mg/L),were used to treat the female adults of T.urticae by the method of slide dipping,and the enzyme activities of glutathione S-transferases (GSTs),carboxylesterases (CarE),and multifunctional oxidases (MFO) were measured and analyzed after treatment for 3,6,12,24,and 48 h.The results showed that after treatment with LC₁₀ and LC₃₀,the activities of CarE,GSTs,and MFO in female adult mites of T.urticae increased in varying degrees compared with the control.The activities of CarE and MFO (except 3 h) in LC₃₀ treatment were higher than that in LC₁₀ treatment,and the activity was the largest within 12~24 h;the GSTs activity in LC₁₀ treatment was significantly (P＜0.05) higher than that in LC₃₀ treatment at 3 h,6 h,and 12 h,and reached the maximum at 24 h.It showed that the spider mite had the strongest detoxification metabolism to Matrine within 12~24 h.With the increase of matrine concentration,the activities of CarE and MFO increased,while the activity of GSTs did not change significantly.