8个饲用燕麦品种萌发期耐盐性综合评价

Comprehensive evaluation of salt tolerance of 8 forage oat varietiesSiriguleng

摘要:

为探究不同饲用燕麦品种间的耐盐性强弱,以8个饲用燕麦品种为试验材料,以蒸馏水为对照(CK)、设25、50及75 mmol/L碳酸氢钠( NaHCO3)盐胁迫处理,测定各处理燕麦的发芽率、发芽势、发芽指数等指标,分析不同浓度的盐胁迫对燕麦种子萌发的影响。以鉴定指标相对值作为耐盐性评价指标,在主成分分析的基础上,构建供试燕麦品种耐盐性评价指标体系,并结合加权隶属函数法综合评价各燕麦品种的耐盐性能。结果表明,随着盐浓度的增加供试品种的发芽率、种子活力都存在下降趋势,其中不同燕麦品种在50 mmol/L盐胁迫下相对发芽率存在显著差异;25 mmol/L浓度下相对活力指数均显著低于CK (P＜0.05) 。利用主成分分析将 6 项评价指标转换为2 个综合指标( Z1、Z2),构建出不同燕麦品种萌发期耐盐性评价体系。在 Z1、Z2权重的基础上,结合加权隶属函数法评价出供试材料种子萌发期耐盐性强弱为甜燕 1 号＞ 牧德乐＞贝勒＞ 燕王＞ 莫妮卡＞枪手＞骏马＞梦龙。

Abstract:

In order to explore the salt-tolerance degree and screen the salt tolerance indexes of different forage oat varieties,the osmotic stress of 25 mmol/L,50 mmol/L,and 75 mmol/L sodium bicarbonate (NaHCO3)was used as treatment,and the distilled water was taken as control,we used eight different oat varieties as test materials to measure the germination rate,germination potential and germination index and to analyze the effects of different concentrations of alkaline salt stress on the germination of oat seeds;Taking the relative value of appraisal index as the evaluation of salinity tolerance,the evaluation index system of salinity tolerance of tested oat varieties was constructed on the basis of principal component analysis,and the salinity tolerance of oat varieties was comprehensively evaluated with the method of weighted membership function.The results showed that seed vigor and germination rate decreased with the increase of salt concentration,and the relative germination rates of oat varieties were significantly different under the salt stress of 50 mmol/L;at the concentration of 25 mmol/L,the seed vigor index was significantly lower than CK (P＜0.05).Six evaluation indexes were converted into two comprehensive indexes (Z1 and Z2) by principal component analysis,and the evaluation system of salt tolerance of different oat varieties during germination was constructed.Based on the weight of Z1 and Z2 and the weighted membership function method,we evaluated the salt tolerance of the tested materials at the germination stage:.The results of this study provided a theoretical basis for the evaluation of salt tolerance of oat,the breeding of salt-tolerant varieties and the study of its mechanism.