Abstract:The alpine grassland of Qilian Mountains is an important part of grassland in northern China.The serious soil degradation of that area in recent years leads to a significant decline in grass yield and quality.The chlorophyll content of forage plants can effectively reflect the growth status of vegetation.Traditional methods for chlorophyll determination is not only time-consuming,but also could cause a certain degree of damage to grassland vegetation.Therefore,the use of hyperspectral technology to monitor plant quality and degradation status of grassland in a real-time manner would be very important.The ASD ground spectrometer was used to collect the spectral data from plant community of the alpine grassland in the Eastern Qilian Mountains.Person correlation coefficient method,PCA principal component analysis and VIP variable projection importance method were used to screen the chlorophyll in plant community of the alpine grassland.The original spectral band and vegetation index of the chlorophyll inversion model were established using multiple stepwise regression and multiple linear regression methods.Our results showed that the reflectivity of the original spectral bands at 384 nm,528 nm,and 721 nm and the chlorophyll correlation coefficient of the alpine grassland plant community are high.A total of 522 original spectral bands were screened as multiple stepwise regression variables.NDVI670,VARI,PSRI,ARVI,RGI,GI,OSAVI,GNDVI are significantly correlated with chlorophyll,and the correlation between vegetation index and chlorophyll was superior to the single-band original spectrum.An inversion model was developed using the original spectral reflectance and vegetation index of the sensitive band ,particularly the original spectral multiple stepwise regression model showed the highest accuracy (R2=0.889) with excellent model test results (R2=0.916 1,RMSE=0.05).