Abstract:This study aimed to explore the effects of different nitrogen levels on the yield and nitrogen use efficiency of Leymus chinensis (Trin.) grown in artificial sandy grassland,together with the change in soil total nitrogen.Four different N levels (100,200,300 and 400 kg/hm2) were applied with nil-N as the control (CK).The result showed that along with the increased nitrogen levels,the yield of Leymus chinensis (Trin.) initially increased then became steady,with the highest yield observed at a nitrogen rate of 309.66 kg/hm2.The regression equation of hay yield against nitrogen application was y=-0.073x2+45.21x+3 808.The crude protein content of Leymus chinensis (Trin.) plants increased along with the increased nitrogen levels.The highest nitrogen use efficiency was found at N3.Total soil nitrogen content,the contribution rate of nitrogen,nitrogen absorption rate were significantly positively correlated with the yield of Leymus chinensis (Trin.).Nitrogen application could improve the crude protein level of Leymus chinensis hay,and the level of nitrogen application affected the amount of residual nitrogen in soil.In this experiment,the optimum yield of Leymus chinensis hay was observed at 309.66 kg/hm2,which can be used as the best fertilization amount for artificial Leymus chinensis grassland in Horqin sandy land.