燕麦根腐病病原真菌分离鉴定及致病性研究

Isolation,identification and pathogenicity of thepathogenic fungi of root rot in oats

摘要:

采集甘肃省通渭县华家岭燕麦主产区燕麦根腐病病株,用组织分离法分离致病菌,通过柯赫氏法则验证其致病性,结合形态学与分子生物学方法鉴定病原菌,以明确引起甘肃省燕麦根腐病病原菌种类,为该病害的诊断和防治提供依据。结果表明:从燕麦病根分离并选取的具有代表性的5株真菌,Y2为茄病镰刀菌(Fusarium solani)、Y5为禾谷镰刀菌(F.gramineae)、Y13为串珠赤霉菌(Gibberella moniliformi)、Y18为G.acuminata、Y31为燕麦镰刀菌(F.avenaceum)。致病性测定表明5株真菌均为燕麦根腐病的致病菌,以禾谷镰刀菌致病力最强,茄病镰刀菌致病力最弱,病情指数分别为79.69与47.00。其中茄病镰刀菌、G.acuminata、串珠赤霉菌引起燕麦根腐病为国内首次报道。

Abstract:

The pathogens causing oat root rot in Gansu province were investigated to provide a basis for the diagnosis and control of the disease.The root rot disease of oat in Huajialing oat producing area,Tongwei county,Gansu province,was collected.The results showed that five representative fungi isolated from the roots with root rot were selected and identified according to the results of morphology and molecular biology.Y2 was Fusarium solan,Y5 was F.gramineae,Y13 was Gibberella moniliformi,Y18 was G.acuminata and Y31 was F.avenaceum.The pathogenicity test showed that all the five fungi were pathogenic bacteria associated with oat root rot disease,and disease index ranged from 47.00~79.69,with F.gramineae being the most pathogenic and F.solan being the least pathogenic.Our results reported for the first time in China that the oat root rot could be cause by F.solan,G.acuminata and G.moniliformi.This study laid a foundation for the study of the aetiology of oat root rot disease and provided a theoretical basis for the prevention and treatment of oat root rotdisease.