Abstract:In this experiment,alfalfa was mixed with bromus inermis,kentucky bluegrass and tall fescuesat in ratios of 1∶1 (mark as A1,B1,C1),1∶2 (mark as A2,B2,C2) and 2∶1 (mark as A3,B3,C3),respectively.Using the monoculture of alfalfa and grass species as the control,the effects of alfalfa mixed with different perennial grasses on soil enzyme activity were studied.The results showed that mixed sowing increased the activity of soil alkaline phosphatases and invertase in 0~20 cm soil layer compared with alfalfa monoculture,and increased urease activity in the top 20 cm soil layer and all enzyme activities in the 20~40 cm soil layer relative to the grass monoculture.Among all treatments,the mixed cropping of alfalfa and bromus inermis had the highest soil urease and catalase activity,while the mixture of alfalfa and tall fescuesat had the highest activity of alkaline phosphatases.Soil invertase activity was found highest in the mixture of alfalfa and tall fescuesat in the top 20 cm and in the mixture of alfalfa and kentucky bluegrass for the 20~40 cm soil layer.Among the treatments with different ratio of alfalfa to grass within the species combination,the highest soil urease and catalase activity was found in the 0~20 cm and 20~40 cm soil layers for the A2,B3 and C3 treatments,and the highest invertase activity was found in A1,B1 and C3 treated soil.The highest activity of alkaline phosphatases was found in 0~20 cm soil layer for C2 treatment and 20~40 cm soil layer for C3 treatment.