甘肃省胡枝子属植物的遗传多样性分析

Analysis of the genetic diversity of Lespedeza in Gansu

摘要:

采用SSR分子标记技术,研究甘肃胡枝子属10个种的遗传多样性和遗传结构,并利用10对紫花苜蓿和4对山生柳SSR引物对胡枝子进行了通用性分析,筛选出7对重复性高、条带清晰的引物,获得105个SSR多态性位点,多态性达83%。引物平均等位基因数为1.91,平均有效等位基因数为1.51。各引物的Shannon指数的多态性平均为0.44。用非加权平均聚类法(UPGMA)进行聚类,将甘肃省胡枝子属植物大体分为3类群。多花胡枝子、美丽胡枝子相聚,再与阴山胡枝子归为一类群;截叶胡枝子、尖叶胡枝子、达乌里胡枝子、牛枝子和绒毛胡枝子为第2类群;二色胡枝子和细梗胡枝子聚为第3类群。结果表明SSR标记可作为胡枝子属种间及种内遗传分析的有力工具,胡枝子种质材料具有较高的遗传多样性。

Abstract:

Genetic diversity and genetic structure of Lespedeza from Gansu were analyzed by SSR markers.Fourteen highly stable and repeatable SSRs (selected from 10 SSRs of Medicago and 4 SSR of Salix oritrepha) were used and a total of 126 alleles,of which 105 were polymorphic (83% of the total),were detected.The average number of alleles of primers was 1.91 and the average number of effective alleles was 1.51.At the species level,the average polymorphism of Shannon index (I) was 0.44.A dendrogram based on genetic distance was constructed by UPGMA method.Ten Lespedeza species in Gansu province were divided into 3 groups.Lespedeza floribunda Bunge,L.formosa (Vog.) Koehne were grouped together with L.inschanica (Maxim.)Schindl.Lespedeza cuneata (Dum.-Cours.) G.Don,L.cyrtobotrya Miq clustered,L.cdaurica (Laxm.) Schindl and L.potaninii V.Vassil were classified as the second group.Lespedeza bicolor Turcz and L.virgate (Thunb.) Dc were gathered as the third group.The results showed that Lespedeza had high genetic diversity,and SSR markers could be used as a powerful tool for the genetic analysis of inter- and intra-species of Lespedeza.