To screen alfalfa cultivars suitable for planting along Huaihe River and its surrounding areas in Anhui Province,this study investigated the production performance and overwintering rate of seven different alfalfa cultivars originated from the United States with different autumn dormancy grades over three years between 2018~2020 in field.Hay yield (DM),crude protein content (CP),neutral detergent fiber content (NDF),acid detergent fiber content (ADF) and the relative feeding value (RFV) were determined.Phenology and overwintering rate were recorded.Our results showed that the higher the fall dormancy level,the earlier plants turn green in spring.Alfalfa cv.6010,Juneng 6 and Juneng 995 had the best overwintering ability.Alfalfa cv Leaf-free had the lowest contents of NDF (37.80%) and ADF (30.03%) over three years,while its mean RFV values was highest (161.78%),being significantly higher than the other alfalfa cultivars.Alfalfa cv Leaf-free was the best giving its CP content and hay yield,followed by WL343 and WL656.Those three cultivars would be suitable for planting along Huaihe River and its surrounding areas in Anhui Province.