芒萁黄酮类物质成分鉴定及体外抗氧化活性研究

Identification of flavonoid components fromDicranopteris pedata and its antioxidant activity in vitro

摘要:

芒萁是一种在南方地区分布广泛的药用草,但目前利用不广。为充分研究其药理基础,应用超声辅助法提取芒萁黄酮,通过聚酰胺柱层析配合梯度洗脱对其进行分离纯化,鉴定其主要成分后,进行体外抗氧化实验。结果表明:芒萁黄酮的提取得率为13.42%,纯化所得50%醇洗物部分的总黄酮含量最高,达到76.25%,主要含有阿福豆苷(2.155%)、木犀草素-5-O-葡萄糖(1.462%)、异槲皮苷(0.856%)。芒萁黄酮对羟基(OH)、1,1-二苯基-2-三硝基苯肼(DPPH)、2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)自由基的IC50值分别为:1 079.01、248.74和50.14 μg/mL。芒萁黄酮含量高,抗氧化活性优良,极具开发价值。

Abstract:

Dicranopteris pedata is a medicinal herb widely distributed in south China,but not widely utilized.The crude flavonoids were extracted using ultrasonic-assisted extraction,separated and purified by polyamide resin column chromatography with gradient elution.After the main components were identified by HPLC,in vitro antioxidant activity was investigated.The results showed that the yield of flavonoids from Dicranopteris pedata was 13.42%,and the total flavonoids content of the purified 50% ethanol gradient elution was the highest,reaching 76.25%,mainly containing afzelin (2.155%) and luteolin-5-O-glucoside (1.462%),isoquercitrin (0.856%).The scavenging values IC50 of the flavonoids from Dicranopteris pedata on hydroxyl (·OH),DPPH,ABTS+ free radicals were 1 079.01,248.74 and 50.14 μg/mL respectively.Dicranopteris pedata had a high content of flavonoids and excellent antioxidant activity,which is of great development value.