不同促生菌混配对燕麦生长及品质的影响

Effects of different combinations of growth promotingbacteria on growth and quality of oat

摘要:

以前期研究筛选出的具有固氮、溶磷、分泌激素和生防特性的6株耐低温植物根际促生菌(G12、I1、Fn1、mF-4、mJ-2、Bn12)为研究材料,采用盆栽法,设不同菌液用量,T1(1∶0∶0∶0)、T2(0∶1∶0∶0)、T3(3∶5∶1∶1)、T4(4∶4∶1∶1)和T5(CK)共计5个处理,测定不同比例下燕麦生长指标和营养指标,研制对燕麦生长和品质影响最佳的复合微生物菌剂。结果表明:与T5相比,各促生菌组合处理均能促进燕麦生长和提高燕麦品质。燕麦株高、茎粗分别增加了5.18%~14.15%和27.37%~40.51%;鲜重、干重分别增加了17.20~28.95 g和3.10~5.95 g;粗蛋白、粗脂肪蛋白分别增加了6.42%~29.57%和0.65%~2.06%;中性洗涤纤维、酸性洗涤纤维含量以及相对饲用价值分别增加了1.19%~4.07%、0.17%~4.63%和0.99%~6.17%。灰色关联度综合分析表明,不同比例菌株制作的菌剂加权关联度:T3(0.171)＞T2(0.168)＞T4(0.143)＞T1(0.137)＞T5(0.081)。处理T3(固氮、溶磷、分泌IAA和产铁载体4种特性的促生菌体积比是3∶5∶1∶1)对燕麦生长和品质的影响均明显高于其他处理和CK,是后续研制微生物菌肥较为理想的配方。

Abstract:

Six strains (G12,I1,Fn1,mF-4,mJ-2,Bn12) with characteristics of nitrogen fixation,phosphate solubilization,hormone secretion and iron-producing carriers were selected as materials for the study based on former research with each basin being added in proportion to a total of 12 mL liquid bacteria,namely: treatment T1(1∶0∶0∶0),treatment T2(0∶1∶0∶0),treatment T3(3∶5∶1∶1) and treatment T4(4∶4∶1∶1).The results showed that the growth and quality of oat were improved by all the combinations of growth-promoting bacteria compared with T5(CK).Specifically,plant height and stem diameter increased by 5.18%~14.15% and 27.37%~40.51% ;fresh weight and dry weight increased by 17.20 g~28.95g and 3.10 g~5.95 g;crude protein and crude fat increased by 6.42% ~ 29.57% and 0.65% ~ 2.06% respectively.The value of neutral detergent fiber,acid detergent fiber and relative feeding value increased by 1.19%~4.07%,0.17%~4.63% and 0.99%~6.17% respectively.The comprehensive analysis of grey relational grade showed that the weighted relational grade of inoculum produced by different proportion of strains was T3(0.171) ＞T 2(0.168) ＞ T 4(0.143) ＞T 1(0.137) ＞T5(0.081) .Treatment T3(nitrogen fixation: phosphate solubiliation: IAA secretion: siderophore is 3∶5∶1∶1) was superior to other treatments and T5 in growth and quality.The experiment implied that T3 is an ideal formulation for the development of microbial fertilizer.