亚致死剂量三唑锡对二斑叶螨Tetranychusurticus解毒酶系的影响

Effect of sublethal doses of azocyclotin on detoxifica-tion enzymes in Tetranychus urticus

摘要:

为明确三唑锡对二斑叶螨体内解毒酶系的影响,在室内采用叶片喷雾法,以亚致死剂量LC₁₀(0.159 mg/L)和LC30(0.247 mg/L)三唑锡处理二斑叶螨敏感品系后,测定不同时间段其体内羧酸酯酶(CarEs)、谷胱甘肽S-转移酶(GSTs)和多功能氧化酶(MFOs)的酶比活性,为二斑叶螨对三唑锡的抗性机制研究提供科学依据。结果如下:二斑叶螨用LC10和LC30剂量的三唑锡处理3、6、12、24和48 h后,与对照相比,其体内GSTs、CarEs和MFOs酶比活性,随时间增长,均不同程度升高且明显高于对照。LC₃₀处理组GSTs酶比活性明显高于LC₁₀处理组,在6和24 h时达到最大;LC₃₀处理组的CarEs酶比活性高于LC₁₀处理组;LC₃₀处理组的MFOs酶比活性高于LC₁₀处理组且LC₁₀处理组CarEs和MFOs酶比活性均在48 h时达到最大。3种解毒酶的酶比活性随三唑锡浓度的增加而升高。

Abstract:

To determine the effect of azocyclotin on the detoxification enzymes and resistance in Tetranychus urticae,cowpea leaves were sprayed to provide the sublethal doses LC₁₀(0.159 mg/L) and LC₃₀(0.247 mg/L) to the strain sensitive strain of Tetranychus urticae for different time period 3、6、12、24 and 48 h.The changes of CarEs,glutathione-S-transferases (GSTs) and multifunctional oxidases (MFOs) enzymes in T.urticae were analysed in vivo.The results are as follows:after 3、6、12、24 and 48 h treatment with LC₃₀ and LC₁₀,compare with that control,the enzyme activities of GSTs,CarEs and MFOs increased over time.All of them increased to different degrees and were significantly higher than that of control.Enzyme activity in LC₃₀ treatment group was significantly higher than that in LC₁₀ treatment group,reached the maximum at 6 h and 24 h.Enzyme activity of CarEs in LC₃₀ was higher than that in LC₁₀;Enzyme activity MFOs in LC₃₀ was higher than in LC₁₀.With the LC₁₀ treatment group the enzyme activity of CarEs and MFOs reached the maximum at 48 h.The activity of three detoxification enzymes increased with the increase of azocyclotin concentration.