‘兰引3号’结缕草足球场丛枝菌根真菌多样性及菌群结构分析

Diversity and community structure analysis of arbuscular mycorrhizal fungi in Zoysia japonica ‘Lanyin No.3’football fields

摘要:

为筛选合适的 AMF 菌剂 ，以解决华南地区常见的‘ 兰引 3 号 ’结缕草(Zoysia japonica ‘Lanyin No. 3’)足球场草坪养护管理过程中存在的水肥浪费和潜在的环境问题。本研究运用扩增子技术对 4 个以‘兰引 3 号’结缕草建植的足球场草坪 AMF 进行群落结构和物种鉴定。结果表明，4 个足球场的‘兰引 3 号’结缕草均与 AMF 形成了共生结构，侵染形式以菌丝侵染为主。通过多样性分析发现，在管理强度较低的英东体育场(YD)中，‘兰引 3 号’结缕草根际土壤和根内 AMF 多样性均高于其他管理强度较高的场地。草坪中的 AMF 大部分属于球囊霉属(Glomus)，其次为盾巨孢囊霉属(Scutel- lospora)以及无梗囊霉属(Acaulospora)；可见，球囊霉属真菌是组成高效 AMF 菌剂的重要菌种资源， 能为‘兰引 3 号’结缕草足球场草坪保水、保肥等菌根技术的发展提供保障。

Abstract:

Arbuscular mycorrhizal fungi(AMF)can form mutualistic symbiosis with plant roots，which is benefi‐ cial to plant growth and development. In order to screen the AMF suitable for Zoysia turf，amplicon technique was used in the molecular identification of the AMF community structure and species of four football field turf plants with Zoysia japonica‘Lanyin No. 3’. The result showed that Z. japonica‘Lanyin No. 3’in all football fields formed sym‐ biotic structures with AMF，and the main form of infection was hyphal infection. The AMF communities in all the lawns were dominated by Glomus，followed by Scutellospora and Acaulospora. In conclusion，the AMF inoculants with the Glomus fungus as the main component could provide guarantee for the constrution and development of my‐ corrhizal technologies such as water and fertilizer conservation of Z. japonica‘Lanyin No. 3’football lawn.