白三叶不同部位水浸提液对草地早熟禾的化感作用

Allelopathy effect of aqueous extracts from different parts of white clover on kentucky bluegrass

摘要:

为探究白三叶(Trifolium repense)不同部位水浸提液对草地早熟禾的化感作用，采用培养箱测定法，研究白三叶根、茎和叶水浸提液对草地早熟禾种子萌发(发芽率、发芽势、发芽指数和活力指数)和幼苗生长(幼苗长、幼苗鲜重和幼根长)的影响。结果表明：白三叶不同部位的水浸提液对草地早熟禾种子萌发均有抑制作用，抑制程度由大到小依次为根＞茎＞叶。不同质量浓度白三叶根、茎、叶水浸提液对草地早熟禾的化感作用强弱顺序基本一致，均为 0. 02 g/mL 浓度水浸提液的抑制作用最弱， 0. 08 g/mL 浓度水浸提液的抑制作用最强。相同质量浓度下，白三叶 3 个部位化感的综合效应依次为： 叶＞根＞茎。总体来看，白三叶不同部位水浸提液处理对草地早熟禾种子萌发和幼苗生长多表现为化感抑制作用，呈明显的浓度效应，即随着水浸提液浓度的升高而抑制作用逐渐增强，且草地早熟禾种子萌发对白三叶化感响应的敏感性要强于其幼苗生长。

Abstract:

In order to investigate the allelopathic effects of water extracts from different parts of white clover on Kentucky bluegrass，the effects of water extracts from roots，stems and leaves of white clover on seed germination (germination rate，germination potential，germination index and vitality index) and seedling growth (seedling length，seedling fresh weight and young root length)of kentucky bluegrass were studied by incubator method. The re‐ sults showed that the water extracts from different parts of white clover inhibited seed germination of kentucky blue‐ grass，and the order of inhibition was root ＞stem＞leaf. The results also showed that the allelopathic effects of wa‐ ter extracts from roots，stems and leaves of white clover at different concentrations on kentucky bluegrass were in the same order，with 0. 02 g/mL water extract having the weakest inhibitory effect and 0. 08 g/mL water extract having the strongest inhibitory effect. Among them，0. 02 g/mL water extracts from roots，stems and leaves could promote seed germination and seedling growth of Kentucky bluegrass. Under the same mass concentration，the comprehen‐ sive effect of allelopathy of three parts of white clover was in the order of leaf water leachate ＞ root water leachate ＞ stem water leachate. In general，water extracts from different parts of white clover showed allelopathic inhibition on seed germination and seedling growth of Kentucky bluegrass. The allelopathic inhibition showed obvious concentra‐ tion effect，that is，with the increase of the concentration of water extract，the inhibition gradually increased. Be‐ sides，the seed germination of kentucky bluegrass was more sensitive to allelopathy of white clover than its seedling growth.