As an important large park green space in Shanghai，Chenshan Botanical Garden not only satisfies people's recreation and leisure needs，but also has the core function of introducing and conserving the genetic germ‐ plasm resources of east China flora. The contradiction between limiting the use of weed chemical herbicides and the spread of weeds is prominent. In order to solve the contradiction，sample method was used to investigate the species， occurrence period and distribution of weeds in the botanical garden. It was found that there were 108 species of weeds in the primary plant conservation area of Chenshan Botanical Garden，49 species of weeds in the central artificial gar‐ den，and 19 species of weeds under the two types of vegetation. Compositae and gramineae accounted for the major‐ ity of weeds，accounting for 15. 2% and 11. 6%，respectively. The species of weeds mainly occurred in March of spring and summer and July of summer and autumn，and the occurrence time of weeds was consistent with the annual occurrence regularity of "dicotyledonous weeds ‐ monocotyledonous weeds ‐ dicotyledonous weeds" and "perennial weeds ‐ annual weeds ‐ perennial weeds". On the basis of investigation and analysis of weed occurrence，according to the weed harm grade，through physical and chemical comprehensive control measures to compare the control effect of the two background turf grass species，it is concluded that the grass with zoysia 'Belair' as the background can achieve the best weed control effect in July. This systematic weed control strategies could provide valuable reference methods for other botanical gardens and parks .