The purpose of this study was to investigate the effects of initial moisture content and sodium diacetate on the quality of fermented total mixed ration(FTMR). Three moisture contents(50%,60%,65%)and four so- dium diacetate levels(0,0. 2%,0. 4%,0. 8%)were designed for a two-factor completely randomized trial. Sensory evaluation,conventional nutrient contents,fermentation parameters and mycotoxins of FTMR were analyzed 35 days after ermentation. The results showed that moisture content,sodium diacetate and their interactions had significant ef- fects on the formation of FTMR organic acid(P<0. 05). With the increase of moisture content,pH value decreased significantly,and the content of lactic acid and acetic acid increased significantly. The crude protein,neutral deter- gent fiber,acid detergent fiber and mycotoxins decreased slightly,with no significant difference(P>0. 05). The ad- dition of sodium diacetate significantly reduced the content of ammoniacal nitrogen/total nitrogen and mycotoxins(P <0. 05). The pH was the lowest with 0. 2% of sodium diacetate ,and the crude protein and neutral detergent fiber were higher. It can be concluded that appropriate moisture content and sodium diacetate can improve the fermentation quality of FTMR,reduce the loss of dry matter,and inhibit the proliferation of mycotoxins. Under the conditions of this study,60% moisture and 0. 2%~0. 4% sodium diacetate were the suitable combination to achieve the desired fermentation quality.