Rotational grazing in the Karst area of South China can effectively improve the utilization rate of grass- land and obtain higher economical grazing returns. Making a scientific rotation grazing plan is very important to lengthening the years of grassland use，reducing the proportion of weeds and increasing the weight of livestock. In this study，the effects of different number of sheep，grazing days and rotational grazing frequency on grass yield， weed proportion and body weight of sheep were investigated by selecting the mixed perennial grass/white clover grassland in Zhuopu，Guizhou Province and taking local free grazing as a control. The results of the 2 -year experi- ment showed that：1)using total grassland biomass，regeneration grass yield，edible forage biomass and the propor- tion of grass and bean as evaluation indexes，the rotation plan in which，six sheep averagely grazed for twelve days in each paddock and the rotation frequency was three times，was optimum；2)with weed biomass control and weed fre- quency as evaluation indexes，the rotation plan that ten sheep averagely grazed for nine days in each paddock and the rotation frequency was three times was the best；3)with the monthly weight gain of sheep as the evaluation index， the rotation plan that six sheep averagely grazed for twelve days in each paddock and the rotation frequency was three times was the best. Integrated considering the effects of rotation grazing on grassland productivity，weeds control and body weight gain of sheep，the study suggested that six sheep averagely grazed for twelve days in each paddock in the initial period of grass/white clover mixed artificial grassland in the Karst area of South China，and the frequency of ro- tational grazing was 3 times.