燕麦孢囊线虫生防真菌TL16发酵条件优化

Optimization of solid fermentation for biocontrol fungus TL16 against Heterodera avenae

摘要:

为探明燕麦孢囊线虫生防真菌长枝木霉菌株 TL16 固体发酵最佳产孢条件，采用正交设计对其发酵基质、碳源、氮源及无机盐最优配比及最佳培养条件进行优化。结果表明：菌株 TL16 最佳培养基质为麦麸∶玉米粉∶玉米秸秆粉=3∶1∶2(重量比)，添加 2% 乳糖、4% 蛋白胨和 4% 硫酸锰作为碳源、氮源和无机盐，初始固料∶水为 1∶1(重量比)，发酵温度 28 ℃，接种量为 15%，黑暗条件下发酵 8 d 时，产孢量最大，为 2. 48×10¹⁰ cfu/g。

Abstract:

In order to establish the optimal conditions of solid fermentation for the spore production of biocontrol fungus TL16 against Heterodera avenae ，the ratio of fermentation substrates，ratios of carbon，nitrogen，and culture conditions were optimized using orthogonal design. The results showed that the optimal medium substrate for strain TL16 was wheat bran：corn flour：corn stalk powder with ratio of 3∶1∶2. In addition，2% lactose，4% peptoneand 4% manganese sulfate were optimal compositions to provide sources of carbon，nitrogen and minerals. The optimal ratio of initial solid materials and water was 1∶1，with 15% inoculation. The maximal sporulation quantity was 2. 48×10¹⁰ cfu/g following fermentation for 8 d at 28 ℃ in dark.