The change of land use structure is of profound significance to the spatio-temporal change and sus- tainable development of regional ecosystem service value. Based on the data of land use structure in the water supply area of the Yellow River in Gannan from 1980 to 2017,the dynamic attitude of land use and the modified equivalent factor method of ecosystem service value were used to analyze the ecosystem service value and its temporal and spa- tial changes. The results showed that grassland became the largest land use type with the ecological service value of ¥ 183. 18×108 ,followed by woodland,wetland and cultivated land. In 37 years,the ecosystem service value of woodland only increased by ¥ 29. 46×108 ,while the others all decreased to different degrees. The total value of eco- system services in the water supply area of the Yellow River in Gannan increased from 328. 50×108 yuan in 1980 to 329. 63×108 yuan in 2017,basically maintaining a stable state and experiencing a two -period fluctuation of "decline and growth" during the period. The value of different ecosystem service functions fluctuated,among which,the value of regulation function was up to 211. 86×108 yuan,support function 77. 82×108 yuan,supply function 21. 39×108 yuan,and cultural service 18. 56×108 yuan. The regional difference of ecosystem service value was significant. Maqu county ranked first with 113. 55×108 yuan of ecological service value,Xiahe County ranked second and Lintan County had the lowest ecological service value,which was only 10. 26×108 yuan. The adjustment function value of each region is the highest. Maqu County assumes the important ecological adjustment function of the study area with the value of 79. 77×108 yuan. It is of great significance to implement forest protection projects and grassland ecologi- cal compensation mechanisms,broaden the livelihoods of residents in the study area,reduce dependence on local ecol- ogy,coordinate regional planning and joint prevention and control,inherit and promote the Yellow River culture,so as to better build local development.