8份多叶羽扇豆种质幼苗对干旱胁迫的生理响应及抗旱性评价

Evaluation of physiological response and drought resistance of seedlings of eight Lupinus polyphyllus accessions to drought stress

摘要:

【目的】探究干旱胁迫对不同多叶羽扇豆种质叶片的生理特性影响。【方法】以 8 份多年生多叶羽扇豆种质作为试验材料，以 30% 的 PEG‐6000 溶液模拟干旱胁迫，测定干旱胁迫后 8 份多叶羽扇豆种质的生理指标。【结果】冰岛种质相对抗旱性较强，渗透调节物质含量较高，MDA 含量相对较低，活性氧积累量趋于低水平，抗氧化酶活性最高；美国 B 种质相对抗旱性较弱，渗透调节物质含量最低， MDA 含量最高，活性氧积累量最高，抗氧化酶活性趋于最低水平。【结论】采用隶属函数方法综合评价表明，多叶羽扇豆的抗旱能力排序为：冰岛＞美国 A＞江苏＞德国＞临夏＞陕西＞酒泉＞美国 B。本研究结果为多叶羽扇豆的种植推广提供理了论依据。

Abstract:

【Objective】 To explore the effects of drought stress on physiological characteristics of leaves of differ‐ ent lupine germplasm.【Method】 Eight Lupinus polyphyllus lindl germplasm were used as experimental materials， and 30% PEG‐6000 solution was used to simulate drought stress，and the physiological indexes of eight germplasm were compared after drought stress.【Result】 The results showed that the germplasm with higher relative drought re‐ sistance had higher content of osmotic regulatory substances，lower content of MDA，lower accumulation of reactive oxygen species，and the higher activity of antioxidant enzymes than those of the relative weak drought resistance germ‐ plasm. By contrast，the germplasm with relatively weak drought resistance had the lowest content of osmotic regula‐ tory substances，the highest content of MDA，the highest accumulation of reactive oxygen species and the lowest ac‐ tivity of antioxidant enzymes.【Conclusion】 The results of the membership function method showed that the drought resistance of Lupinus polyphyllus lindl was ranked as' Iceland '＞ ' America B '＞ 'Jiangsu '＞ 'Germany'＞ ' linxia'＞ 'Shanxi '＞' Jiuquan '＞'America B'. The results of this study provide a theoretical basis for the cultivation and promo‐ tion of Lupinus polyphyllus lindl.