黑果枸杞PYL转录因子家族鉴定与生物信息学分析

Identification and bioinformatics analysis of PYL transcription factor family of Lycium ruthenicum

摘要:

【目的】基于黑果枸杞（Lycium ruthenicum）果实发育过程中转录组测序结果，对黑果枸杞 PYL 转录因子家族成员进行鉴定。【方法】将黑果枸杞果实发育过程中鉴定出的 PYL 转录因子进行理化性质、多序列对比、保守基序、果实发育过程中的表达谱分析。【结果】与黑果枸杞果实发育相关的 9 个 PYL 转录因子氨基酸数目介于 166~255，分子量 18 229. 42~24 689. 97 D，理论等电点 5. 33~7. 14， 不稳定系数为 35. 08~44. 05，家族成员中含有 1 个稳定碱性蛋白、4 个稳定酸性蛋白、4 个不稳定酸性蛋白。【结论】通过分析基因表达谱发现，LrPYL7 和 LrPYL8 成员在黑果枸杞果实发育全时期中（绿熟期、变色期、完熟期）均呈现高表达，可能对该过程具有重要调控作用，这将为黑果枸杞及 PYL 转录因子的进一步研究奠定基础。

Abstract:

【Objective】 Based on the transcriptome data of Lycium ruthenicum during fruit development，the members of PYL transcription factor family in L. ruthenicum were identified.【Method】 The identified PYL transcrip- tion factors were further used to determine physical and chemical properties，multi-sequence comparison，conservative motif and expression levels during fruit development.【Result】 Results indicated that the number of amino acids of 9 PYL transcription factors related to the fruit development of Lycium ruthenicum was 166~255，the molecular weight was 18 229. 42~24 689. 97 D，the theoretical PI was 5. 33~7. 14，and the instability index was 35. 08~44. 05. The Family members contain one stable alkaline protein，four stable acidic proteins and four unstable acidic proteins. 【Conclusion】 By analyzing the gene expression patterns，it was found that LrPYL7 and LrPYL8 members were highly expressed in the whole fruit development period （green ripening period，discoloration period and full ripening period） of L. ruthenicum，indicating that they may play an important role in regulating this process，which would lay a foundation for further research on transcription factors of Lycium ruthenicum and PYL.