4种宿主植物对根内球囊霉和摩西球囊霉的扩繁效果及菌根接种效应

Propagation effect and mycorrhizal inoculation effect of 4 host plants on 2 AM fungi

摘要:

【目的】拓宽丛枝菌根（Arbuscular mycorrhizal，AM）真菌扩繁的宿主植物范围，推进 AM 真菌在退化草地生态修复中的应用进程。【方法】以阿坝垂穗披碱草（Elymus nutans cv. Aba）、川草 2 号老芒麦（Elymus sibiricus cv. Chuancao No 2）、北林 201 紫花苜蓿（Medicago sativa cv. Beilin201）和白三叶草（Trifolium repens）为宿主植物，对根内球囊霉（Glomus intraradices，GI）和摩西球囊霉（Glomus moss- eae，GM）进行了扩繁，并测定了 4 种宿主的菌根接种效应。【结果】4 种宿主植物均能与 GI 和 GM 识别共生形成菌根，菌根侵染率均为：白三叶草＞北林 201 紫花苜蓿＞阿坝垂穗披碱草＞川草 2 号老芒麦； 栽培两个月后，用白三叶扩繁的 GI 组和 GM 组的孢子密度均最高。接种 GI 和 GM 后，4 种宿主植物的株高、生物量和叶绿素含量均不同程度增加。【结论】多年生禾本科牧草和豆科牧草均可作为扩繁根内球囊霉和摩西球囊霉的宿主植物，以白三叶草为宿主时扩繁效果最佳。

Abstract:

【Objective】 In order to expand the host plant range for propagation of arbuscular mycorrhizal fungi， 【Method】 Perennial gramineous forages Elymus Nutans 'Aba '，Elymus Sibiricus 'Chuancao No 2' and leguminous for- age Medicago sativa 'Beilin 201' and Trifolium repens were selected as host plants of Glomus intraradices and Glomus Mosseae，and effects of mycorrhizal inoculation on four hosts was measured.【Result】 The results showed that four host plants can be infected by both Glomus intraradices and Glomus Mosseae，and the infection rates of four host plants were Trifolium repens＞ Medicago sativa‘Beilin201’ ＞ Elymus nutans‘Aba’＞ Elymus sibiricus‘Chuancao No 2’. After two months of cultivation，the spore density of treatment propagated by Trifolium repens was the highest. Af- ter inoculation with Glomus intraradices and Glomus Mosseae，plant height，biomass and chlorophyll content of the four host plants were increased.【Conclusion】 Both perennial gramineous and legume forages could be used as host plants of Glomus intraradices and Glomus Mosseae，and Trifolium repens was the best host for propagation.