【Objective】 Through the rotation of oats with rapeseed,Vicia sativa,highland barley and barley,the effects on soil enzyme activity and soil microbial quantity were studied.【Method】 Therefore,a rotational cropping ex- periment was conducted in the field planting oat for 3 years in Shandan Army Horse Farm. Common vetch,oat+ common vetch,rape,nakedbarley,barley and oat were planted respectively.【Result】 The results showed that soil en- zyme activity declined with the increasing soil depth. Crop rotation significantly increased soil catalase,invertase and alkaline phosphatase activities compared with continuous cropping. The enzyme activity of oat-legume rotation was much higher than that of oat-grass,among which,oat-common vetch rotation had the most significant effect. At flow- ering stage,the activities of catalase,invertase and alkaline phosphatase in the 0~20 cm soil layer increased 55. 92%, 20. 36% and 51. 47%,respectively. Cropping rotation also significantly increased the number of soil microorganisms. The treatment of oat-common vetch gave the greatest increment,the number of soil bacteria in 0~10 cm,10~20 cm and 20~30 cm soil layers increased 23. 34%,16. 51% and 38. 92%,respectively,compared with continuous crop- ping (P<0. 05). The number of fungi increased 110%,135. 29%,and 150%,and the number of actinomycetes in- creased 112. 65%,107. 41%,and 175. 38%,respectively.【Conclusion】 The rotation of oat-legume increased soil en- zyme activity and number of microorganisms significantly more than that of oat-grass. In the production of oat in Qil- ian Mountains,the rotation of oat-common vetch is expected to repair continuous cropping obstacles and improve the soil environment.