NaCl胁迫下25份饲用型小黑麦种子的萌发特性及耐盐性评价

Evaluation of germination characteristics and salt tolerance of 25 forage triticale seeds under NaCl stress

摘要:

【目的】探究不同饲用型小黑麦（×Triticosecale）种质耐盐性强弱。【方法】对比分析了 25 份饲草型小黑麦种质材料在 150 mmol/L NaCl 胁迫下的发芽势、发芽率、发芽指数、芽长、根长和活力指数，并对其耐盐性进行了综合评价。【结果】150 mmol/L NaCl 胁迫下，25 份小黑麦种质材料的发芽率、 发芽势、发芽指数、芽长、根长和活力指数较对照（CK）均显著降低（P＜0. 01），且材料间差异极显著（P ＜0. 01）。经隶属函数法和系统聚类法综合评价，可将 25 份材料划分为 3 类：第 1 类有 3 份种质材料（冀饲草一号 、石大一号和鉴 -18），属于高耐盐型 ；第 2 类有 17 份种质材料（X-515、X-509、X522、 TWXB-6、X-143、TWXB-3、鉴 -46、TWXB-11、X-520、XJ- 小黑麦 、TWXB-4、X-516、 TWXB-2、HN-小黑麦、TWXB-8、鉴-47 和晋饲草一号），属于中度耐盐型；第 3 类有 5 份种质材料 （TWXB-1、TWXB-7、TWXB-5、TWXB-9、TWXB-10），属于盐敏感型。【结论】冀饲草一号的综合隶属排名最高，较耐盐胁迫。本研究可为耐盐饲用型小黑麦新品种选育及利用提供试验依据。

Abstract:

【Objective】 The aim of the study is to investigate the salt tolerance of different forage triticale germ‐ plasm.【Method】 The germination potential，germination rate，germination index，shoot length，root length and vigor index of 25 forage triticale materials under 150 mmol/L NaCl stress were compared and analyzed.【Result】 The ex‐ periments showed that the germination rate，germination potential，germination index，shoot length，root length and vigor index of 25 materials were significantly lower （P＜0. 01） at 150 mmol/L NaCl concentration compared with the control （CK），and the difference between materials was highly significant （P＜0. 01）. The 25 materials could be divided into three types after comprehensive evaluation by the membership function method and systematic clustering method. The first type had three germplasm materials （Ji Forage No. 1，Shi Da No. 1 and Jian‐18），which belonged to high salt tolerance type. The second type was made up of 17 germplasm materials （X-515，X-509，X522， TWXB-6，X-143，TWXB-3，Jian-46，TWXB-11，X-520，XJ-triticale，TWXB-4，X-516，TWXB-2， HN- triticale，TWXB-8，Jian-47，and JIN Forage No. 1），which were moderately salt-tolerant. The third type consisted of five germplasm materials （TWXB-1，TWXB-7，TWXB-5，TWXB-9，and TWXB-10），which were salt-sensitive.【Conclusion】 This study could provide a basis for the selection and utilization of new salttolerant forage triticale varieties.