叠氮化钠对燕麦种子萌发及幼苗生长的影响

The effect of sodium azide on seed germination and seedling growth of oat

摘要:

【目的】明确不同 NaN₃浓度及处理时间对燕麦种子萌发及幼苗生长的影响。【方法】筛选半致死剂量，以 2 个燕麦品种爱沃和青永久 709 子为材料，设置不同 NaN₃浓度（0、5、10、15、20 mmol/L） 和处理时间（1、2、3 h），测定种子萌发活力及幼苗生长指标。【结果】 NaN₃浓度是影响燕麦种子萌发及幼苗生长的最主要因子。燕麦种子的相对发芽率、相对发芽势及相对发芽指数随 NaN₃浓度的升高而显著下降，当 NaN₃浓度为 10 mmol/L、处理时间为 1 h 时，青永久 709 的相对发芽率为 51. 08%、相对致死率为 47. 92%；同一浓度下处理 2 h 时，爱沃的相对发芽率为 51. 47%、相对致死率为 48. 53%。NaN₃ 浓度超过 10 mmol/L，两品种的相对致死率均大于 60%，最高浓度（20 mmol/L）下种子完全丧失发芽能力。NaN₃处理还显著抑制了幼苗生长，同一条件下青永久 709 的出苗率、根长和苗高受到抑制的程度重于爱沃。【结论】青永久 709 和爱沃的半致死剂量分别为 10 mmol/L 处理 1 h 和 10 mmol/L 处理 2 h，青永久 709 对 NaN₃处理比爱沃更敏感。

Abstract:

【Objective】 Inorder to clarify the effects of different NaN₃ concentrations and treatment time on oat seed germination and seedling growth of oat，and select half lethal dose.【Method】 The seeds of two varieties （Ever- leaf and Qingyongjiu 709） were used as materials. Different NaN₃ concentrations （0，5，10，15，20 mmol/L） and treat- ment time （1，2，3 h） were set to analyze the seed germination activity and seedling growth after treatment. 【Result】 The results showed that NaN₃ concentration was the main factor affecting oat seed germination and seedling growth. The relative germination rate，relative germination potential and relative germination index of oat seeds de- creased significantly with the increase of NaN₃ concentration. When the NaN₃ treatment of 10 mmol/（L·1 h），the relative germination rate of Qingyongjiu 709 was 51. 08% and the relative lethal rate was 47. 92%. When treated for 2 h at the same concentration，the relative germination rate of Everleaf was 51. 47% and the relative lethal rate was 48. 53%. When the concentration of NaN₃ was greater than 10 mmol/L，the relative mortality of the two varieties was greater than 60%. The seeds completely lost germination ability at the highest concentration （20 mmol/L）. NaN₃ treatment also significantly inhibited seedling growth. The emergence rate，root length and seedling height of Qingyongjiu 709 were more restricted than that of Everleaf under the same condition.【Conclusion】 The half lethal treatment combinations of Qingyongjiu 709 and Everleaf were 10 mmol/L，1 h and 10 mmol/L，2 h，respectively. Qingyongjiu 709 was more sensitive to NaN₃ treatment than Everleaf.