单宁酸对红色和绿色豌豆蚜酶活性的影响

Effects of tannic acid on the enzymatic activity of red and green pea aphids (Acyrthosiphon pisum)

摘要:

【目的】明确单宁酸对红色和绿色豌豆蚜酶活性的影响 。【方法】设不同浓度单宁酸（1 mg/L、3 mg/L、5 mg/L）分别饲喂两种色型的 5 d 龄豌豆蚜，处理不同时间后测定豌豆蚜体内超氧化物歧化酶（SOD）、过氧化物酶（POD）、羧酸酯酶（CarE）及细胞色素 P450s 酶（CYP450）活性。【结果】豌豆蚜取食不同浓度单宁酸后，体内 4 种酶的响应程度不同。红色型豌豆蚜 CarE 与 CYP450 酶活性均在 24 h 处理的单宁酸浓度为 3 mg/L 时达最大值 ，分别为（0. 029±0. 002）μmol/（μg·min）和（0. 006± 0. 000 9）μmol/（μg·min），和对照间差异显著（P＜0. 05）；绿色型豌豆蚜 SOD 与 POD 酶活性均在 72 h 处理的单宁酸浓度为 3 mg/L 时最高，分别达（0. 028±0. 004）μg/mg 和（1. 679±0. 172）μg/（mg·min），和单宁酸浓度为 5 mg/L 的处理间差异显著（P＜0. 05）。【结论】红色和绿色豌豆蚜取食不同浓度单宁酸后，体内 SOD、POD、CarE 和 CYP450 酶活性均有不同程度的影响；相同处理时间，同种酶的活性在红绿色型豌豆蚜内的升高或降低程度不同；同种色型豌豆蚜取食不同浓度单宁酸后，体内不同酶的活性变化不同。

Abstract:

【Objective】In order to determine the effects of tannic acid on the enzymatic activity of red and green Acyrthosiphon pisum.【Method】 In this study，different concentrations of tannic acid （1 mg/L，3 mg/L and 5 mg/L） were fed with two color types of 5 d-aged pea aphid. SOD，POD，CarE and P450s enzyme activities in pea aphid were measured following different treatment times.【Result】 The four enzymes responded differently to the different concentrations of tannic acid. With 24 h treatment，the maximum enzyme activities of CarE enzyme and P450 were 0. 029±0. 002 μmol/（μg·min） and 0. 006±0. 000 9 μmol/（μg·min），respectively，which were significantly different （P＜0. 05），when tannic acid concentration was 3 mg/L. The enzyme activities of SOD and POD of green A. pisum were the highest and reached 0. 028±0. 004 μg/mg and 1. 679±0. 172 μg/（mg·min），respectively，when tannic acid concentration was 3 mg/L at 72 h. These were significantly different from tannic acid concentration of 5 mg/L treatment （P＜0. 05）.【Conclusion】 The different concentrations of tannic acid affected the activities of SOD，POD，CarE and P450 in red and green A. pisum . With the same treatment time，the activity of the same enzyme increased or decreased to different degrees in the red and green type A. pisum. The activities of different enzymes in different concentrations of tannic acid were different in the same type of A. pisum.