外源赤霉素浸种对盐胁迫下高羊茅种子萌发和幼苗生长的影响

Effects of soaking seeds with exogenous gibberellinon seed germination and seedling growth of tall fescue under salt stress

摘要:

【目的】 探究外源赤霉素浸种对盐胁迫下高羊茅种子萌发和幼苗生长的影响。【方法】 以高羊茅火凤凰2 号为材料，采用纸上发芽法，设置20 个处理，用0、100、150、200、300 mg/L GA 溶液浸泡高羊茅种子2 d，然后将其分别置于含0、100、200、250 mmol/L NaCl 溶液的培养皿中进行萌发试验，并测定种子萌发和幼苗生长的相关指标。【结果】 在100 mmol/L 盐胁迫下，能缓解盐胁迫进而促进种子萌发和幼苗生长的最佳GA 浓度为200 mg/L，种子萌发各项指标优于0 mg/L GA 处理，在200、250mmol/L 盐胁迫下，能缓解盐胁迫从而促进种子萌发和幼苗生长的最佳GA 浓度为150 mg/L，种子萌发各项指标略优于0 mg/L GA 处理。【结论】 模糊数学隶属函数法对不同浓度GA 溶液浸种缓解盐胁迫能力综合分析证明，能够缓解盐胁迫并促进种子萌发和幼苗生长的较佳赤霉素浓度为150 mg/L。

Abstract:

【Objective】 The study was carried out to investigate the effect of exogenous gibberellin infusion onseed germination and seedling growth of tall fescue under salt stress.【Method】 In this experiment， tall fescue "FirePhoenix 2" was used as experimental material， and paper germination method was adopted. Twenty treatmentgroups were set up to soak tall fescue seeds with 0，100，150，200 and 300 mg/L GA solution for 2 days， and thenplaced them in petri dishes containing 0，100，200 and 250 mmol/L NaCl solution for germination test， and the relatedindexes of seed germination and seedling growth were determined respectively.【Result】 The analysis of relatedindexes showed that under 100 mmol/L salt stress， the optimal GA concentration for alleviating salt stress and thuspromoting seed germination and seedling growth was 200 mg/L， and all the indexes of seed germination were betterthan those of the 0 mg/L GA treatment， and under 200 and 250 mmol/L salt stress， the optimal GA concentrationfor alleviating salt stress and thus promoting seed germination and seedling growth was 150 mg/L，and all the indexesof seed germination were slightly better than those of the 0 mg/L GA treatment.【Conclusion】 The comprehensiveanalysis of salt stress relieving ability of different concentration GA solution by fuzzy mathematical membership functionmethod showed that the best concentration of gibberellin which could relieve salt stress and promote seed germinationand seedling growth was 150 mg/L.