首页 > 过刊浏览>2024年第44卷第4期 >189-196. DOI:10.13817/j.cnki.cyycp.2024.04.021
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Pseudomonas simiaeMHR6产胞外多糖条件优化及抗氧化活性
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作者:
作者单位:

1.甘肃农业大学草业学院，草业生态系统教育部重点实验室，甘肃省草业工程实验室，中‐美草地畜牧业可持续发展研究中心，甘肃 兰州 730070 ;2.金昌市金川区农业技术推广服务中心，甘肃 金昌 737100

作者简介:

刘晓婷（1996-），女，甘肃榆中人，博士研究生。E-mail：1184877443@qq.com

中图分类号:

S154.3

基金项目:

甘肃省教育科技创新专项（GSSYLXM-01）；甘肃省林业与草原局草原生态修复治理科技支撑项目（GSAU-TSYF-2021-011）


Optimization of culture conditions and antioxidantactivity of exopolysaccharides produced byPseudomonas simiae MHR6
Author:
Affiliation:

1.College of Grassland Science,Gansu Agricultural University,Key Laboratory for Grassland Ecosystem,Minis⁃try of Education,Grassland Engineering Laboratory of Gansu Province,Sino-U.S.Centers for Grazing Land Eco⁃system Sustainability,Lanzhou 730070 ,China ;2.Agricultural Technology Extension Service Center of Jinchuan Dis⁃trict,Jinchang City,Jinchang 737100 ,China

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    摘要:

    【目的】 研究培养条件对Pseudomonas simiae MHR6 生长及胞外多糖（EPS）产量的影响，并探究其抗氧化活性，为新型农业生物制剂的开发利用提供可靠的理论依据。【方法】 以EPS 产量为指标，通过单因素试验对菌株MHR6 产EPS 条件进行优化，并对EPS 的羟基自由基（OH-）、超氧阴离子（O2·-）和过氧化氢（H2O2）的清除作用进行了研究。【结果】 经优化后，MHR6 产EPS 的最佳培养条件是：培养时间5 d、温度28 ℃ 、初始pH 值8、接种量3%。当EPS 质量浓度为1. 0 mg/mL 时，其OH-、O2·-和H2O2 的清除率分别为56. 94%、64. 08% 和31. 69%。【结论】 最佳培养条件下EPS 产量达到最大值，为6. 16 mg/mL，比优化前提高了24. 9%。抗氧化实验结果表明MHR6 所产的EPS 具有良好的抗氧化活性。

    Abstract:

    【Objective】 In this paper，the effects of culture conditions on the growth of Pseudomonas simiaeMHR6 and the production of exopolysaccharides（EPS） were studied， and the antioxidant activity of P. simiaeMHR6 was investigated，providing a reliable theoretical basis for the development and utilization of new agriculturalbiological agents.【Method】 With the yield of EPS as the index，the EPS production conditions by MHR6 was optimizedby the single factor test. Subsequently，the antioxidant activity was determined by OH- ，O2·- and H2O2 scavengingrate of EPS.【Result】 The results showed that after optimization， the optimal culture conditions for MHR6 toproduce EPS were as follows：culture time 5 d，temperature 28 ℃ ，initial pH value 8，inoculation amount 3%. Whenthe mass concentration of EPS was 1. 0 mg/mL，the scavenging rates of OH-，O2·- and H2O2 were 56. 94%，64. 08%and 31. 69% respectively.【Conclusion】 Under the optimal culture conditions，EPS yield reached the maximum valueof 6. 16 mg/mL，which was 24. 9% higher than that before. Antioxidant activity results showed that EPS had strongantioxidant activity.

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刘晓婷,张琛,韩江茹,李晓林,姚拓.Pseudomonas simiaeMHR6产胞外多糖条件优化及抗氧化活性[J].草原与草坪,2024,44(4):189-196

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  • 收稿日期:2022-10-17
  • 最后修改日期:2022-10-29
  • 在线发布日期: 2024-09-26
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